EGF ELISA Kit

Catalog No. ABIN5521805

This Human EGF ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human EGF. This ELISA Kit has been cited in1 publication.

Quick Overview for EGF ELISA Kit (ABIN5521805)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.906 pg/mL - 250 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details EGF ELISA Kit

Minimum Detection Limit: 3.906 pg/mL

Purpose: For quantitative detection of EGF in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of EGF. No significant cross-reactivity or interference between EGF and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between EGF and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 2.344 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

References for EGF ELISA Kit (ABIN5521805)

De Iuliis, Marino, Caruso, Capodifoglio, Flati, Marynuk, Marricareda, Ursi, Lanuti, Talora, Conti, Martinotti, Toniato: "Autophagy processes are dependent on EGF receptor signaling." in: Oncotarget, Vol. 9, Issue 54, pp. 30289-30303, (2018) (PubMed).

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF

Background: EGF(Epidermal growth factor)/URG/HOMG4/Beta-Urogastrone

UniProt: P01133

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation